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Diabetes 50 : 11101118 Yakar S et al

G.ClarkeG.et al

Following collection, adipose tissue explants were rinsed in phosphate-buffered saline (PBS) (P4474, Sigma-Aldrich, Pozna, Poland) supplemented with 1% penicillin/streptomycin (P/S) (P4333, Sigma- Aldrich, Pozna, Poland) to prevent microbial contamination and then transferred into transport medium consisting of Dulbeccos Modified Eagles Mediumlow glucose (DMEM-LG containing 1000 mg/L of glucose) (D9443, Sigma- Aldrich, Pozna, Poland) supplemented with 1% P/S

Curr Drug Targets 20:12171226

Cu 2+ , which released from decomposed CCF in an acidic environment, triggered GSH exhaustion (Figure 5A) and Cu + -mediated Fenton-like reaction, avoiding the potential OH elimination