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Normally, FOXO4 prevents p53 from triggering apoptosis in aging cells, allowing them to persist and contribute to age-related diseases (1)

The seeded scaffolds were washed with PBS, fixed in 4% paraformaldehyde for 30 min, and then rinsed to eliminate the PFA

By maintaining neural circuit integrity, reinforcing neuroplasticity, and mitigating stress-induced impairments, the combination of exercise and polyphenols provides a robust defense against cognitive decline and mood disorders

Consistent with the results observed in in vivo experiments, GHK-Cu significantly restored Nrf2 expression in the cytoplasm and reduced it in the nucleus while significantly reducing Keap1 levels in the cytoplasm compared with the CSE-exposed cells without GHK-Cu (Figures 6DF)