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Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h

it also involves sustaining system homeostasis by negative feedback mechanisms (Joseph and Whirledge, 2017)

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49 The second one is safety, which is a critical issue

Regulation of 25-hydroxyvitamin D3 1alpha-hydroxylase gene expression by parathyroid hormone and 1,25-dihydroxyvitamin D3

Histological analysis confirmed that miR-ADSCs enhanced eNOS and VEGFa expression in penile tissues, reduced apoptosis, and preserved smooth muscle integrity, suggesting that miR-423-5p inhibition in ADSCs enhances their therapeutic efficacy for DMED by simultaneously upregulating the eNOS and VEGFa pathways [102]