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5 Enzymes and metabolites related to glycolysis in ischemic stroke The glycolytic pathway is regulated by three key rate-limiting enzymes: Hexokinase (HK), Phosphofructokinase (PFK), and Pyruvate kinase (PK), which catalyze irreversible reactions (18)

Given the extended literature reporting the influence of pubertal hormones on HPA-axis [49], redox regulation [50, 51] and brain maturation [11], we explored in a secondary hypothesis whether the aforementioned associations were related to pubertal status and gonadal hormones (estradiol and testosterone)